Bio-One Franchise Review 2026: Costs, Fees, News, Average Revenues and/or Profits

Bio-One provides specialized cleanup of biohazards, including death-related scenes, hoarding, and drug-related cleanup, along with mold and odor remediation, work driven by events rather than ordinary consumer demand. Against a $134,645 to $221,095 investment range, the 113 franchisees who reported 12 months of revenue averaged $372,962 in 2025 (down 4.1% from 2024), producing a 2.1:1 sales-to-investment ratio. Results vary widely: the top quartile averaged $802,263 versus just $80,429 for the bottom quartile, a 10.0x gap. Death-related jobs generated nearly half of total revenue (49.7%), followed by hoarding (23.4%). Among the franchisees that submitted P&L reports, average EBITDA was 16.1% of revenue after the 7.5% royalty (down from 18.7% in 2024), which works out to an estimated $60,084 per franchisee and a payback period of 2.2 to 3.7 years. Franchised outlets fell from 152 to 144 in 2025.

Ambrosio’s Take is a new feature on all Franchise Chatter FDD Talk posts based on the 2026 FDD.

In this exclusive Franchise Chatter FDD Talk post, you’ll learn the following:

Section I – Estimated initial investment (franchise costs) for a Bio-One franchise, based on Item 7 of the company’s 2026 FDD

Section II – Initial franchise fee, royalty fee, and marketing fee for a Bio-One franchise, based on Items 5 and 6 of the company’s 2026 FDD

Section III – Number of franchised and company-owned Bio-One outlets at the start of the year and the end of the year for 2023, 2024, and 2025, based on Item 20 of the company’s 2026 FDD

Section IV – News updates on the Bio-One franchise opportunity

Section V – Presentation and analysis of Bio-One’s financial performance representations (average revenues and/or profits), based on Item 19 of the company’s 2026 FDD, including information on the:

• 2025 average, median, high, and low annual revenues, by quartile, for the 113 Bio-One franchisees who have reported 12 months of revenue or more

• 2025 average (as a percent of revenue) COGS, facilities and supplies, payroll and personnel, marketing and advertising, insurance, legal and accounting, auto and travel, local tax and licensing, other business expenses, meals and entertainment, bank charges, other income/expenses, total cost, and adjusted EBITDA for the 61 Bio-One active franchisees in good standing who submitted profit and loss reports to Bio-One

• 2025 adjusted revenue and percent of total revenue per job category (death-related bio, hoarding, non-death related bio, mold/odor, and drug) for the 113 Bio-One franchisees who have reported 12 months of revenue or more

Section VI – Key ratios, comparables, computations, and analyses for the Bio-One franchise opportunity (exclusive content for Platinum subscribers)

Section I – Bio-One Franchise Costs

• Bio-One franchise costs, based on Item 7 of the company’s 2026 FDD:

• Initial Franchise Fee:  $60,000
• QSP Package:  $39,500
• Technology Startup Fee:  $1,595
• Annual Conference Registration Deposit:  $2,000
• Rent and Rental Improvements:  $0 to $1,500
• Furniture, Fixtures, and Equipment:  $0 to $2,000
• Computer Hardware, Software, and Other Electronics:  $800 to $7,800
• Initial Training Expenses:  $0 to $3,500
• OSHA Training Fee:  $2,000
• OSHA Training Expenses:  $0 to $3,500
• Local Marketing:  $9,000 to $15,000
• Business Vehicle:  $0 to $45,000
• Business Vehicle Tax, Title, and License:  $0 to $4,000
• Business Vehicle Insurance:  $1,000 to $1,200
• Certifications:  $2,500 to $3,500
• Insurance and Professional Services:  $1,250 to $4,000
• Additional Funds for 3 Months:  $15,000 to $25,000
• Total Estimated Bio-One Franchise Costs:  $134,645 to $221,095

Section II – Bio-One’s Initial Franchise Fee, Royalty Fee, and Marketing Fee

• Bio-One’s initial franchise fee, royalty fee, and marketing fee, based on Items 5 and 6 of the company’s 2026 FDD:

• Initial Franchise Fee:  $60,000

• Royalty:  the greater of the applicable Minimum Royalty or 7.5% (Percentage Royalty) of Gross Sales

• Minimum Royalty:  the Minimum Royalty will never be less than $750 during the Initial Term of the Franchise Agreement. It will increase from $750 to $1,500 in the 13th month, and increase again from $1,500 to $2,250 from the 25th month through the remainder of the Initial Term of the Franchise Agreement.

• Marketing Fee:  the greater of $200 or Bio-One’s then-current fee that now is 2% of the Gross Sales per month

• Local Advertising Fee:  the greater of $3,000 or 5% of Gross Sales per month

• Regional Advertising Cooperative Fee:  currently $0.00. If a Regional Advertising Program is established, you may be required to contribute a specified part of your Local Advertising Fee to the Regional Advertising Cooperative.

Section III – Number of Franchised and Company-Owned Bio-One Outlets

Franchised

2023

• Outlets at the Start of the Year:  144
• Outlets at the End of the Year:  146
• Net Change:  +2

2024

• Outlets at the Start of the Year:  146
• Outlets at the End of the Year:  152
• Net Change:  +6

2025

• Outlets at the Start of the Year:  152
• Outlets at the End of the Year:  144
• Net Change:  -8

Company-Owned

2023

• Outlets at the Start of the Year:  0
• Outlets at the End of the Year:  0
• Net Change:  0

2024

• Outlets at the Start of the Year:  0
• Outlets at the End of the Year:  0
• Net Change:  0

2025

• Outlets at the Start of the Year:  0
• Outlets at the End of the Year:  3
• Net Change:  +3

Section IV – News Updates on the Bio-One Franchise

• Five Star Franchising Names Ben Kramer as Brand President of Bio-One

• Bio-One and Lion National Corp. Announce Affiliate Partnership Under OMNIA Partners Contract

• Three Five Star Franchising Brands Named 2025 Top Franchises for Veterans by Entrepreneur

• Bio-One Did Not Rank on Entrepreneur’s 2026 Franchise 500 List

Section V – Financial Performance Representations (Average Revenues and/or Profits) for the Bio-One Franchise (Item 19, 2026 FDD)

• 2025 Performances of Franchisees Who Have Reported 12 Months of Revenue or More (i.e., who have been open for at least 12 months within which to report revenue; as a result, 113 Franchisees’ performance fits within this table, with the excluded franchisees only representing those who were not in operation for each of the 12 months in 2025):

• No Franchisees have been excluded, unless they did not report, in which case Bio-One could not include their data.

Part 1 – Franchisee Average Revenues

Tier 1

• Number of Franchisees:  28
• Average Revenue:  $802,263
• High:  $1,380,424
• Low:  $533,389
• Median:  $709,844
• Number and Percent Above Average:  11/39%

Tier 2

• Number of Franchisees:  28
• Average Revenue:  $393,058
• High:  $498,160
• Low:  $314,948
• Median:  $385,848
• Number and Percent Above Average:  13/46%

Tier 3

• Number of Franchisees:  28
• Average Revenue:  $226,545
• High:  $312,574
• Low:  $148,781
• Median:  $217,488
• Number and Percent Above Average:  13/46%

Tier 4

• Number of Franchisees:  29
• Average Revenue:  $80,429
• High:  $148,510
• Low:  $5,280
• Median:  $73,138
• Number and Percent Above Average:  13/45%

Part 2 – Average Expenses as Percentage of Average Revenue

• The following table reflects the average revenues of those locations who reported profits and losses and expenses to Bio-One through submission of financial reports. The data is as-received from such owners.

• Bio-One received 61 profit and loss reports from franchisees with greater than 12 months of operation. For these, none have been excluded save for those who had not been operational for such time or those for whom data was not received.

Average (as a Percent of Revenue)

• COGS:  9.30%
• Facilities and Supplies:  4.50%
• Payroll and Personnel:  35.20%
• Marketing and Advertising:  10.00%
• Insurance:  3.20%
• Legal and Accounting:  2.10%
• Auto and Travel:  3.80%
• Local Tax and Licensing:  0.00%
• Other Business Expenses:  3.50%
• Meals and Entertainment:  1.50%
• Bank Charges:  0.50%
• Other Income/Expenses:  2.80%
• Total Costs:  76.39%
• Adjusted EBITDA:  16.11%

• The Payroll and Personnel costs reported to Bio-One include owner draws/benefits, and both direct and indirect labor costs.

• The expenses reported to Bio-One reflected in the Bank Charges additionally include bad debt allowances or write-offs.

• The Adjusted EBITDA makes final adjustments to assure that the applicable royalty rate and any other fees paid to Bio-One as the franchisor have been matched to those you will incur under your franchise agreement, even if prior versions may be historically inconsistent on some terms relating to such fees.

• Average reported based upon all 61 active franchisees in good standing who supplied 2025 P&L Reports, operating in 75 locations.

Part 3 – 2025 Average Job Categories Data

Death-Related Bio

• Adjusted Revenue:  $22,546,185
• % of Total Revenue:  49.70%

Hoarding

• Adjusted Revenue:  $10,630,085
• % of Total Revenue:  23.40%

Non-Death Related Bio

• Adjusted Revenue:  $7,735,869
• % of Total Revenue:  17.10%

Mold/Odor

• Adjusted Revenue:  $3,047,454
• % of Total Revenue:  6.70%

Drug

• Adjusted Revenue:  $1,404,982
• % of Total Revenue:  3.10%

• Performances of Franchisees who have reported less than 12 months of revenue have demonstrated such irregular and unpredictable highs and lows in the early stages of their operations that their historical data is not reliably representative of any patterns, and are not reported until after they have completed 12 months of reported revenue from their operations.

• Some outlets have sold this amount. Your individual results may differ. There is no assurance you’ll sell as much.

Section VI – Bio-One Franchise Ratios, Comparables, Computations, and Analyses (Exclusive Content for Platinum Subscribers) ⬇️

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